Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYAISSAADIWDHISESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCGS-PASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 2ORW Chain:B ((2-174)) ------SGKLTVITGPMYSGKTTELLSFVEIYKLGKKKVAVFKPKID-----TMIVSH---GVEAHVIERPEEMRKYIEEDTRGVFIDEVQFFNPSLFEVVKDLLDRGIDVFCAGLDLTHKQNPFETTALLLSLADTVIKKKAVCHRCGEYNATLTLKVAGGEEE------IDVGGQEKYIAVCR-----------

General information: TITO was launched using: RESULT: Template: 2ORW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 850 -111831 -131.57 -681.90 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -131.57 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_2ORW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ORW-query.scw PDB file : Tito_Scwrl_2ORW.pdb: