Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREELKGIFASVFGEKEGLRFFFAPGRVNLIGEHTDYNGGHVFPCALTMGTYAAVAERNDGLVRMYSDNFRNAGIKECSLDDIRYQKEDDWANYPKGVIYEFQQRGYAVPHGFDIVFSGNIPNGAGLSSSASIELLMGVVLQSYFHPEVDALELVKMAQHAENHFIGVNCGIMDQFAIGMGKKHHAMLLNCDTLDYEYSKLNVSGLALVIANTNKKRTLADSSYNTRRQECNDALLDLKKGLDIAALGDIKPSDFDAHSSLIQNETNRRRAKHAVYENHRAIKTAHMFKENNIDEIGQLMKESHLSLKDDYEVTCPELDELVFAAWDHEGVIGSRMTGAGFGGCTISIVKDEFVDDFIQKVGDRYQEKTGLRADFYVADIGEGARELKGE 1FWK Chain:A ((1-296)) MKVRVKAPCTSA----------------NL---------------GVGFDVFGLCLKEPYDVIEVEAIDDKEIIIEV-DDKNIPTDPDKNVAGIVAKKMIDDFNI----GKGVKITIKKGVKAGSGLGSSAASSAGTAYAINELFKLNLDKLKLVDYASYGELASSGA--KHADNVAPAIF----------------------GGFTMVTNYEPLEVLHIPID-----FKLDILIAIPNISINTKEAREILPKAVG--------------LKDLVNNVGKACGMVYALYNKDKSLFGRYMMSDKVIEPVRGK-LIPNYFKIKEEV--KDKVYGITISGSGPS--IIAFPKEEFIDEVENILRDYYEN-------TIRTEVGKGVEVV---

General information: TITO was launched using: RESULT: Template: 1FWK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1821 -149287 -81.98 -504.35 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -81.98 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_1FWK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FWK-query.scw PDB file : Tito_Scwrl_1FWK.pdb: