Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIRNPFKDKYYSHDRRALNMLALRVPGLAFILMIYIASIVLQFVSGGWSILLLYAFTILIAIFALLHWHSYRWVKKRVILYFAVQGLITFALANLMTGFFILVIIGLYAFLIGQIIGMADRRRTFLILYLLLLLVINSAYHLHKGEVLHFIVIAAPIMIVIITYAATFFAQVDEKIKAQLTLERLELAHQQVEQLTLQNERQRMARDLHDTLAQGLVSLNMQLDAIHVHLAKGNTERAKEIIQQSMKRVKSTIADARSAIDDLRS-KSEEIGV-LKERITSLMDHFIESTGMACLL-DYRLHQVLDVRTAENCYYIIGECMTNAAKHAEAKTIWISI---WDDEKGRLHLTVKDNGKGFDVEKGKKKRGHYGLLGIQERVRAINGQFNIKSTKLKGTQIEITVPIQGEMQDE 3EHF Chain:A ((9-193)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RIARDLHDTLGQKLSLIGLKSDLARKLIYKDPEQAAREL--------KSVQQTARTSLNEVRKIVSSMKGIRLKDELINIK-QILEAADIMFIYEEEKWPENISLLNENILSMCLKEAVTNVVKHSQAKTCRVDIQQLWKE----VVITVSDDGT-FKGE-------H-GLLGMRERLEFANGSLHIDTE--NGTKLTMAIP--------

General information: TITO was launched using: RESULT: Template: 3EHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 617 -66652 -108.03 -387.51 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -108.03 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_3EHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EHF-query.scw PDB file : Tito_Scwrl_3EHF.pdb: