Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDEFDLIHSITPRTIHHSSVDVGIGDDAALYTAKHGVQEIVCVDTMVEDVHFKLHYSSPEDIGYKALAVNISDIAAMGGIPKFYLVSLAVPS---KWTESEIKAMYEGMNELAKLYHMDLIGGDTVSTADKLVVTVTVIGEIEKGQACLRSLAKPNDIVFVTGEIGSSAAGLSLLLEETNPQNSSVETDYFIHRHKRPEPRVSVGRLCSIFKRAALNDVSDGLASELNEIAEASCVSIEIVESMLPIHSDLPKLHPNWK-EWALFGGEDFELTGTVSNEEWEVLKQECAALHLPITKIGYVREKTKSKVILKTDQTSMILEKKGYNHFK 5D9U Chain:A ((10-312)) -AEFSIIDQYFNRQ-SHPDVALGIGDDSALITPPPNQQLVICADTLVAGRHFPLE-TSPHAIGWKSVAVNLSDIAAMGAKPHSILLAISLPQVDHEWLEGFSQGIYDCCNQ----FGVALIGGDT-TQGPHLTITVTAMGWIETGKAVLRSGAKVGDYVCVSGQIGDAAYGLQHL-------GHSLQ-----QRLDYPTPRCKLGEELKGLA-SSMIDVSDGLAQDLGHILKASKVGARLILEKLPVDPVLQQIEEQQRWQYALAGGDDYELCFTITPQNYEKLLQK--QLDVKITMIGQIVEQT--KLTFEHLGSDYPLQIHGYQHF-

General information: TITO was launched using: RESULT: Template: 5D9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1690 -171445 -101.45 -573.39 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -101.45 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_5D9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D9U-query.scw PDB file : Tito_Scwrl_5D9U.pdb: