Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKQYWFIILTYIIMQFSALIAIPLLFKFGYAGGQPTDENMLHAQGLWSVISFIACLVVVLLILRTVPKETLRN--GQKDSIGLSILWAIAGFFIAL-FSQGIAGSIEYYVFGIGRESENTQAILDVIQAVPLMIIVSSIVGPILEEIIFRKIIFGALYEKTNFFFAGL----------ISSVIFGIVHADLKHLLLYTAMGFTFAFLYARTKRIWVPIFAHLMMNTFVVIMQLEPVRNYLEQQSTQMQLIIGGLFL 1OY6 Chain:A ((427-575)) -------------------------------------------------------------------PKEATRKSMGQIQGALVGIAMVLSAVFVPMAFFGGSTGAI-YRQFSI-----------TIVSAMALSVLVALILTPALCATMLK--------------FGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLP---------------------------------------

General information: TITO was launched using: RESULT: Template: 1OY6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 133 -26704 -200.78 -238.42 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -200.78 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.046

(partial model without unconserved sides chains): PDB file : Tito_1OY6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OY6-query.scw PDB file : Tito_Scwrl_1OY6.pdb: