Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTWRKSYERWKQTEHLDLELKERLIELEGDEQALEDCFYKDLEFGTGGMRGEIGAGT-NRMNIYTVRKASAGFAAYISKQGEEAKKRGVV----IAYDSRHKSPEFAMEAAKTLATQGIQTYVFDE--LRPTPELSFAVRQLNAYGG-----IVVTASHNPPEYNGYKVYGDDGGQLPPKEADIVIEQVNA-----IENELTITVDEENKLKEKGLIKIIGEDIDKVYTEKLTSISVHPELSEEVDVKVVFTPLHGTANKPVRRGLEALGYK-NVTVVKEQELPDSNFSTVTSPNP-EEHAAFEYAIK--LGEEQNADILIATDPDADRLGIAVKNDQGKYTVLTGNQTGALLLHYLLSEKKKQGILPDNGVVLKTIVTSEIGRAVASSFGLDTIDTLTGFKFIGEKIKEYEASGQYTFQFGYEESYG--YLIGD---FARDKDAIQAALLAVEVCAFYKKQGMSLYEALINLFNEYGFYREGLKSLTLKGKQGAEQIEAILASFRQNPPQKMAGKQVVTAEDYAVSKRTL-LTESKEEAIDLPKSNVLKYFLEDGSWFCLRPSGTEPKVKFYFAVKGSSLEDSEKRLAVLSEDVMKTVDEIVESTAK 3OLP Chain:A ((64-542)) -----------------------------------------VKFGTSGHRGSAGRHSFNEPHILAIAQAIA----------EERAKNGITGPCYVGKDTHALSEPAFISVLEVLAANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPPNGGPADTNVTKVVEDRANALLAGGLQGVKRISLDAA---MASGHVKAV--DLVQPFVEGLADI-VDMAAIQKAGLTLGVDPLGGSGIEYWKRIAEH--YKLNLTLVNDQVDQTFRFMHLDKDGAIRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVT-----PAGLMNPNHYLAVAINYLFQHRPLWG---KDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGL--FDGS----FGFGGEESAGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAARFGAPSYNR----------------LQASATSAQKAALSKLS-PEMVSASTLAGDPITARLTAAPGNGASIGGLKVMT----DNGWFAARPSGTEDAYKIY-----------------------------------

General information: TITO was launched using: RESULT: Template: 3OLP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2647 -135580 -51.22 -299.96 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -51.22 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_3OLP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OLP-query.scw PDB file : Tito_Scwrl_3OLP.pdb: