Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAKTKLVTDRIRLRCMEDRDQATLFGLLFNDPDVMTYYS--GLKDKRQTREWVNWNQRNEKGYGVSLWI--AEDKRTGEFLGQCGIVPQQIENQTVMEIGYMFARRHWGNGYAQEAARACLDYGFNERQFGKMAALIDPGNKASIRVAEKIGMHYSKTIRKWNKPIAVYERKSYN 2ZW5 Chain:A ((12-149)) ------LRTARLELTPLDPAADARHLHHAYGDEEVMRWWTRPACADPAETERYLT---SCAAAPGARLWTIRAPD---GTVPGMAGL----LGGTDVPGLTWLLRRDSWGHGYATEAAAAVVGHALEDGGLDRVEAWIEAGNRRSLAVAARVGL----------------------

General information: TITO was launched using: RESULT: Template: 2ZW5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 649 -81858 -126.13 -610.88 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -126.13 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_2ZW5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZW5-query.scw PDB file : Tito_Scwrl_2ZW5.pdb: