Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGIIGGGSVGLLCAYYLSLY-HDVTVVTRRQEQAAAIQSEGIRLYKGGEEFRADCSA-DTSINSDFDLLVVTVKQHQLQSVFSSLERIGK--TNILFLQNGMGHIHDLKDWHVGHSIYVGIVEHGAVRKSDTAVD-HTGLGAIKWSAFDDAEPDRLNILFQHNHSDFPIYYETDWYRLLTGKLIVNACINPLTALLQVKNGELLTTPAYLAFMKLVFQEACRILKLENEEKAWER----VQAVCGQTKENRSSMLVDVIGGRQTEADAIIGYLLKEASLQGLDAVHLEFLYGSIKALERNTNKVF 2OFP Chain:A ((18-309)) MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVN----LVETDGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGTIEELQN--IQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVLPD--VAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIM---QICEEVAAVIEREGHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVPENTRLFEMVKRKESEY----

General information: TITO was launched using: RESULT: Template: 2OFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1526 -209968 -137.59 -741.93 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -137.59 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_2OFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OFP-query.scw PDB file : Tito_Scwrl_2OFP.pdb: