Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIAISGGGTGGHIYPALAFIKEVQRRHPNVEFLYIGTENGLEKKIVERENIPFRSIEITGFKRKLSFENVKTVMRFLKGVKKSKSYLAEFKPDAVIGTGGYVCGPVVYAAAKMGIPTIVHEQNSLPGITNKFLSKYVNKVAICFEEAKSHFPSEKVVFTGNPRASEVVSIKTGRSLAEFGLSEDKKTVLIFGGSRGAAPINRAVIDMQDVLKTRDYQVLYITGEVHYEKVMNELKSKGAADNMVTKPFLHQMPEYLKAIDVIVARAGATTIAEITALGIPSVLIPSPYVTANHQEVNARSLGQHDAAIVLKETELSGEKLIEALDRIVLNEQTLKEMSERTKSLGVPDAAARLYSVLEELKK 1NLM Chain:A ((8-346)) -RLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWL--GTADRMEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGMGGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGA---FPNAEVV--GNPVRTDVLALPLPQQ--RLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGD-SVTIWHQSGKGSQQSVEQAYAEAGQPQHKVTE-FIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPALFVPFQH-KDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAG--WSRETLLTMAERARAASIPDATERV---------

General information: TITO was launched using: RESULT: Template: 1NLM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1980 -198134 -100.07 -584.47 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -100.07 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_1NLM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NLM-query.scw PDB file : Tito_Scwrl_1NLM.pdb: