Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQEKAIIIGGGPCGLSAAIHLKQIGIDALVIEKGNVVNSIYNY---PTHQTFFSSSEKLEIGDVA--FITENRKPVRIQALSYYREVVKRK-------NIRVNAFEMVRKVTKTQNNTFVIETSKETYTTPYCIIATGYYDHPNYMGVP-GEDLPKVFHYFK--EGHPYFDKDVVVIGGKNSSVDAALELVKSGARVTVLYRGNEYSPSIKPWILPEFEALVRNGT----IRMEFGACVEKITENEVVFRSGEKELITIKND-------------FVFAMTGYHPDHQ--FLEKIGVEIDKETGRPFFN-EETMETNVEGVFIAGVIAAGNNANEIFIENGRFHGGHIAAEIAKRENH 2EQ8 Chain:A ((10-329)) ------IVIGTGPGGYHAAIRAAQLGLKVLAVEAGEVGGVCLNVGCIPT-KALLHAAETLHHLKVAEGFGLKAKPELDLKKLGGWRDQVVKKLTGGVGTLLKGNGVELLRGFARLVGPKEV-EVGGERYGAKSLILATG-SEPLELKGFPFGEDVWDSTRALKVEEGLP---KRLLVIGGGAVGLELGQVYRRLGAEVTLI----EYMPEILPQGDPETAALLRRALEKEGIRVR--------TKTKAVGYEKKKDGLHVRLEPAEGGEGEEVVVDKVLVAVGRKPRTEGLGLEKAGVKVDE---RGFIRVNARMETSVPGVY-----AIGDAARPPLLAHKAMREGLIAAE-------

General information: TITO was launched using: RESULT: Template: 2EQ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1392 10382 7.46 36.43 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 7.46 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.341

(partial model without unconserved sides chains): PDB file : Tito_2EQ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EQ8-query.scw PDB file : Tito_Scwrl_2EQ8.pdb: