Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEETFQLLYDTYHQDLYQFLFYMVKDKNQTEDLLQEVYIRVLNSYHTFE-GRSSEKTWLLSIARHVAIDWFRKQQTIRQRILGTFDWDTQDVRDQQLLPDELAVQHENVREIYAALDQCTIDQRAVIILRFIQGYSIQETAKALRFSESKVKTTQHRGLKVLRKHMELLREELMDDEVRMERRTDKGVVKSTSGS 2Q1Z Chain:A ((26-183)) -EAAFAELFQHFAPKVKGFLMKSGSVASQAEECAQDVMATVWQKAHLFDPSRASVATWIFTIARNRRIDGLRKD---RQPEPEDLFWGPDSEPDQA---DVYEMQQENAR-LGRAIARLPEAQRALIERAFFGDLTHRELAAETGLPLGTIKSRIRLALDRLRQHM-----------------------------

General information: TITO was launched using: RESULT: Template: 2Q1Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 463 -15745 -34.01 -100.28 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -34.01 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_2Q1Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q1Z-query.scw PDB file : Tito_Scwrl_2Q1Z.pdb: