Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIEDLWDQFHQPLKTYI--SHRVNDQS--IVDDLLQIVFMKIQVHLPNLIDEQKIDSWIYRITRNTIIDFYRTKKTSEILPDVLHFNDSAEEENF----TKEATVCIRSTIKRLPEKYREALELTDFQGLSQKELSEKLGISYSGAKSRVQRGRGKLKQLLEGCCHIEADRYGNIVDFRILKE 2Z2S Chain:A ((31-181)) ----ELFQHFAPKVKGFLMKSGSVASQAEECAQDVMATVWQKAHLFDPS---RASVATWIFTIARNRRIDGLRKDRQPE--PEDLFWGPDSEPDQADVYEMQQENARLGRAIARLPEAQRALIERAFFGD----------GLPLGTIKSRIRLALDRLRQ------------------------

General information: TITO was launched using: RESULT: Template: 2Z2S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 362 -17484 -48.30 -131.46 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -48.30 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_2Z2S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z2S-query.scw PDB file : Tito_Scwrl_2Z2S.pdb: