Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRFAIIGTNWITDRFLESAADI--EDFQLTAVYSRSAERAGEFAAKHNAAHAFSDLQEMAASDCFDAVYIASPNALHKEQAVLFMNHGKHVLCEKPFASNTKETEEMISAAKANGVVLMEAMKTTFLPNFKELKKHLHKIGTVRRFTASYCQYSSRYDAFRSGTVLNAFQPELSNGSLMDIGVYCIYPAVVLFGA--PKDVKANGYALSSGVDGEGTVILSYDGFEAVLMHSKISTSYAP-----AEIQGEDGTI-VIDTIHRPERVEIRYRDGRLENIAIPDPK-------PAMFYEAEEFVTLIKENKLESEENTFERSLTTAKIMEEARKQMGIVYPADQA 2O48 Chain:X ((3-332)) -LRWGIVSVGLISSDFTAVLQTLPRSEHQVVAVAARDLSRAKEFAQKHDIPKAYGSYEELAKDPNVEVAYVGTQHPQHKAAVMLCLAAGKAVLCEKPMGVNAAEVREMVTEARSRGLFLMEAIWTRFFPASEALRSVLAQ-GTLGDLRVARAEFGKNLT-----HVPRAVDWAQAGGALLDLGIYCVQFISMVFGGQKPEKISVMGRRHETGVDDTVTVLLQYPGE----VHGSFTCSITAQLSNTASVSGTKGMAQLLNPCWCP--TELVVKGEHKEFLLPPVPKNCNFDNGAGMSYEAKHVRECLRKGLKESPVIPLVESELLADILEEVRRAIGVTFPQD--

General information: TITO was launched using: RESULT: Template: 2O48.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1801 -15133 -8.40 -48.35 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain X : 0.77 3D Compatibility (PKB) : -8.40 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_2O48.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O48-query.scw PDB file : Tito_Scwrl_2O48.pdb: