Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEKLRLYHTNDLHSHFENW------------PKIVDYIEQKRKEHQSDGEETLVFDIGDHLDRFQFVTEATFGKANVDL--LNRLHIDGAAIGNNEGITLPHEELAALYDHAEFPVIVSNLFDKN-GNR--PSWAVPYHIKSLKNGMSIAFLGVTV--------PYYPVYDKLGWTVTDALESIKETILEVKGQADIIVLLSHLGILD----------DQAVAEAVP--EIDVILESHTHHLL----EDGQVV-------------NGVLLASAEKYGHYVGCVEITV-DSVQRSINSKTASV---QNMAEWTGESAETK-------AFLNEKEREAEEKLSDAVAELAQDAEVKWFEESELPLLLAYALKEWCETDISMVNSGVILGPLKAGPVTKLDLHRICPHPINPVAVRLTGEELKETIVHAASEQMEQLRIKGLGFRGEVMGKMVYAGVEVETKRLDDGITHVTRITLNGEDIEKHKQYSVAVLDMFTLGKLFPLIRDAAEKEYFMPEFLRDLLAWKLAQ 1HP1 Chain:A ((8-468)) ---KITVLHTNDHHGHF--WRNEYGEYGLAAQKTLVDGI---RKEVAAEGGSVLLLSGGDI---NTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHE-FDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWAL-FKRQDLK----IAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQE--LQQTEKPDIIIAATHMGHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCKPDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKRVLYTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAAQMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAVA-----QMKPDSGAYP-------QFANVSFVAK---DG--KLNDLKIKGEPVDPAKTYRMATLNFNATG------------------------------

General information: TITO was launched using: RESULT: Template: 1HP1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2339 67187 28.72 169.66 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 28.72 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_1HP1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HP1-query.scw PDB file : Tito_Scwrl_1HP1.pdb: