Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGQTPARHYYKKLVPSLILILNCIQFLSHPTKADPILLAFVFAVYLAFIWIIPYVASTAVSLSIFIGLWLLTDFFWAVSGQEQGAAYFLIVFLMIYAAFRLPSRLSLIFTACLIGGNILLLSSQGGSLNTIISNISIMLGLYVLFSSMRFRREARREAERNHAELAKMHVQLEHAHKELQKAHAELQEASVLSLRYAVLEERTRIARDIHDSIGHELTSVIVQLQSLPYILKSSKEDSEKVIQNVLSVARECLQEVRSVVHQMGRSESMVGLTALRGLIHQVEERSGLHVSLDTAGL----SEESWPPNVS----ETIYRILQEALTNIIRHADASHAAAVISNDKSHLYVTITDDGQFTG-SLTYGFGLTGMKERAEKAGGSLTFSAVQPSGLKIELSLPLMTTNKEQKDEQR 3EHF Chain:A ((9-193)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RIARDLHDTLGQKLSLIGLKSDLARKLIYKDPEQAARELKSVQQTARTSLNEVRKIVS------SMKGIRLKDELIN-------IKQILEAADIMFIYEEEKWPENISLLNENILSMCLKEAVTNVVKHSQAKTCRVDIQQLWKEVVITVSDDGTFKGE-----HGLLGMRERLEFANGSLHIDT--ENGTKLTMAIP-------------

General information: TITO was launched using: RESULT: Template: 3EHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 520 4730 9.10 27.99 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 9.10 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_3EHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EHF-query.scw PDB file : Tito_Scwrl_3EHF.pdb: