Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGELANYKRLEKVGEGTYGVVYKALDLRPGQGQRVVALKKIRLESEDEGVPSTAIREISLLKELKDDNIVRLYDIVHSDAHKLYLVFEFLDLDLKRYME-----GIPKDQPLGADIVKKFMMQLCKGIAYCHSHRILHRDLKPQNLLINKDGNLKLGDFGLARAFGVPLRAYTHEIVTLWYRAPEVLLGGKQYSTGVDTWSIGCIFAEMCNRKPIFSGDSEIDQIFKIFRVLGTPNEAIWPDIVYLPDFKPSFPQWRRKDLSQVVPSLDPRGIDLLDKLLAYDPINRISARRAAIHPYFQES 4EON Chain:A ((1-290)) --GSMENFQKVEKIGEGTYGVVYKARNKLTGE---VVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTE-NKLYLVFEFLSMDLKKFMDASALTGIP------LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPENLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQD-

General information: TITO was launched using: RESULT: Template: 4EON.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -180794 for 2248 contacts (-80.4/contact) + 2D Compatibility (PS) -30032 + (NN) -11067 + (LL) 316 1D Compatibility (HY) -38800 + (ID) 9250 Total energy: -269627.0 ( -119.94 by residue) QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_4EON.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EON-query.scw PDB file : Tito_Scwrl_4EON.pdb: