Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNASLVTAFILTGLPHAPGLDALLFGIFLVVYVLTVLGNLLILLVIRVDSHLHTPMYYFLTNLSFIDMWFSTVTVPKMLMTLVSPSGRAISFHSCVAQLYFFHFLGSTECFLYTVMSYDRYLAISYPLRYTSMMSGSRCALLATGTW----------LSGSLHSAVQTILTFHLPYCGPNQIQHYFCDAPPILKLACADTSANVMVIFVDIGIVASGCFVLIVLSYVSIVCSILRIRTSDGRRRAFQTCASHCIVVLCFFVPCVVIYLRPGSMDAMDGVVAIFYTVLTPLLNPVVYTLRNKEVKKAVLKLRDKVAHPQRK 3RFM Chain:A ((14-174)) -----------------------------LAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADILVGVLAIP---FAITISTGFCAACHGCLFIACFVLVLAQSSIFSLLAIAIDRYIAIAIPLRYNGLVTGTRAAGIIAICWVLSFAIGLTPMLGWNNCGQP--------GCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFAAARRQLKQMESQPLPGERARSTLQKEVHAAKSAAIIAGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLAHTNSVVNPFIYAYRIREFRQTFRKI

General information: TITO was launched using: RESULT: Template: 3RFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -115101 for 955 contacts (-120.5/contact) + 2D Compatibility (PS) -14429 + (NN) -5078 + (LL) 3120 1D Compatibility (HY) -14400 + (ID) 2000 Total energy: -147888.0 ( -154.86 by residue) QMean score : 0.101

(partial model without unconserved sides chains): PDB file : Tito_3RFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RFM-query.scw PDB file : Tito_Scwrl_3RFM.pdb: