Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKTVLTEFILLGLTDVPELQVAVFTFLFLAYLLSILGNLTILILTLLDSHLQTPMYFFLRNFSFLEISFTNIFIPRVLISITTGNKSISFAGCFTQYFFAMFLGATEFYLLAAMSYDRYVAICKPLHYTTIMSSRICIQLIFCSWLGGLMAIIPTITLMSQQDFCASNRLNHYFCDYEPLLELSC--SDTSLIEKVVFLVASVTLVVTLVLVILSYAFIIKTILK---------LPSAQQRT---KAFSTCSSHMIVISLSYGSCM--------FMYINPSAKEGDTFNKGVALLITSVAPLLNPFIYTLRNQQVKQPFKDMVKKLLNL 3RFM Chain:A ((18-305)) ---------------------------------VLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADILVGVLAIP-FAITISTGFCA-ACHGCLFIACFVLVLAQSSIFSLLAIAIDRYIAIAIPLRYNGLVTGTRAAGIIAICW---VLSFAIGLTPMLGWNNCGQP--------GCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFAAARRQLKQMESQPLPGERARSTLQKEVHAAKSAAIIAGL-FALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLAHTNSVVNPFIYAYRIREFRQTFRKIIRS----

General information: TITO was launched using: RESULT: Template: 3RFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -221753 for 1927 contacts (-115.1/contact) + 2D Compatibility (PS) -25815 + (NN) -242 + (LL) 4516 1D Compatibility (HY) -25600 + (ID) 3150 Total energy: -272044.0 ( -141.17 by residue) QMean score : 0.327

(partial model without unconserved sides chains): PDB file : Tito_3RFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RFM-query.scw PDB file : Tito_Scwrl_3RFM.pdb: