Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEKNFWPLGIMSVLIFGLGIVVFLVVFALKNSPKNDLVYFKGHNEVDLNFNAML-KTYENFKSNYRFSVGLKPLTESPKTPILPYFSKGTHGDKKIQENLLNNALILEKSNTLYAQLQPLKPALDSPNIQVYLAFYPSQSQPRLLGTLDCKNACEPLKFDLLEGDKVGRYKILFKFVFKNKEELILEQLAFFK 2X42 Chain:A ((375-440)) ---------------------------------PRYAISILEGIKERGLNFDEELAKTYEDYIKKMRETEEYKPRR-----IIKPKLPENFLSEKEIHKLAKKNDVAVIVISRISGEGYDRKPVKGDFYLSDDETDLIKTVSREFHEQGKKVIVLLNIGSPVEVVSWRDLVDGILLVWQAGQETGRIVADVLTG

General information: TITO was launched using: RESULT: Template: 2X42.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -1117 for 268 contacts (-4.2/contact) + 2D Compatibility (PS) -6261 + (NN) -3195 + (LL) 3172 1D Compatibility (HY) -2000 + (ID) 900 Total energy: -10301.0 ( -38.44 by residue) QMean score : 0.170

(partial model without unconserved sides chains): PDB file : Tito_2X42.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X42-query.scw PDB file : Tito_Scwrl_2X42.pdb: