Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLAIWQIILLVILAACTILDALTLVIGLNFPVITGTLAGIIMGDMVLGLAIGATLQLM----VLGVGTYGGASIPDFTTGAIVGTVFAVLSGQDAEFAIGLA-IPVGLLMVQLDILARFTNTFFLHRIDSNIASGNISAVKRNIWYGALPWALSRAVPVFIMLTFGQSVVDFILNEIPEWLMGGLRVAGGILPVVGIAILLRYLPTNKFVAYLIIGFVAAAYLKVPMLGVALIGVALAIIYFKQNFKNPVAAGANGAALVGEENEDGEYED 4S2A Chain:A ((141-278)) --------------------------------------AGIITAEMEY-VAIRENLRREQDRPCVRDGEDFGASIPDFVTPEFVRQ----------EIARGRAIIPANINHGELEPMAIGRN--FLVKINANIGN---STVADEV--DKLVWATRWGADTVMDLSTGRNI-----HNIRDWIIRNSSVPIGTVPI-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4S2A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -96173 for 844 contacts (-113.9/contact) + 2D Compatibility (PS) -12869 + (NN) 2910 + (LL) 12308 1D Compatibility (HY) -6400 + (ID) 2200 Total energy: -102424.0 ( -121.36 by residue) QMean score : 0.126

(partial model without unconserved sides chains): PDB file : Tito_4S2A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4S2A-query.scw PDB file : Tito_Scwrl_4S2A.pdb: