Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDYNHYFHFPREEWRKLEVSKDQILTAEELEEIRGLNDRISLQDISEIYLPLIKLIAIQYHEAIFIHGEKMEYLKKKESRAPFIIALAGSVAVGKSTTARVFKLMLDRWFSKTRQVELVTTDGFLYPNKVLEERGIMDKKGFPESYDRDRFAKFLTDLKANKEDVEIPLYSHFTYDVL-DETRVIHNPDIVIIEGINVLQA------DQHESLFPSDFFDFSVYMDANEADIKKWYLERFFMLRETAFQDESSYFHPYTKISKQEAETFALGVWDTINGVNLKENIEKTKYRADLVLQKGTDHLISDIYLRK 4NE2 Chain:A ((30-334)) ------YLQFNRHQWAALR-----TLTEDEITRLKGINEDLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKSTTARVLQALLSRW-PEHRHVELITTDGFLHPNSVLKERGLMKKKGFPQSYDMHRLVKFVSDLKSGVPQATAPVYSHLIYDVIPDGDKTVAQPDILILEGLNVLQSGMDYPHDPHH-VFVSDFVDFSIYVDAPEELLKSWYINRFLKFREGAFTDPDSYFHNYAKLSKEEAVDIATSLWNEINLMNLKENILPTRERASLIMTKSANHSVNQVRLRK

General information: TITO was launched using: RESULT: Template: 4NE2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -160887 for 2281 contacts (-70.5/contact) + 2D Compatibility (PS) -31505 + (NN) -12882 + (LL) 484 1D Compatibility (HY) -32400 + (ID) 7150 Total energy: -244340.0 ( -107.12 by residue) QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_4NE2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NE2-query.scw PDB file : Tito_Scwrl_4NE2.pdb: