Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLVNMKQLLEVAKENKFAVGAFNVADSNFLRVVVEEAEKNNAPAIIAVHPTELDFTKDDFFQYV-----LARIKNSPVPFVLHLDHGDNMGDVMRAVRCGFSSVMIDGSLLPFEENIRVTKEVVDVCHKLGVSVEGELGTIGKTGNSIEGGVSEIIYTKPEEAEEYISRTGVDTLAVAIGTAHGIYPKDKEPKLRLDILKEIKALVNIPLVLHGGSANPDAEIAAAVEIGIQKVNISSDYKYAFYKKCREILST-TELWDANAIYPDCIDAAKEVVKYKMGLFESIGQVEKYQQAKTTAWRSELI 4TO8 Chain:A ((3-279)) -LVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAPVILGVSEGAARYM-SGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFTSVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGG--DDVV--ADGIIYADPKECQELVEKTGIDALAPALGSVHG-------PKLGFKEMEEIGLSTGLPLVLHGGTGIPTKDIQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKIKEF----------------------

General information: TITO was launched using: RESULT: Template: 4TO8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -164399 for 2284 contacts (-72.0/contact) + 2D Compatibility (PS) -28977 + (NN) -12270 + (LL) 2208 1D Compatibility (HY) -18800 + (ID) 5250 Total energy: -227488.0 ( -99.60 by residue) QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_4TO8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TO8-query.scw PDB file : Tito_Scwrl_4TO8.pdb: