Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNLNEKQLITEIVGLARSQGLTVHSENAQLNETGMDFQVVFAKDDTGMPWVLRKPRRSDVVERASAEGITLAFLRANLTADVPDWRIHTPELIAYPMLKGTPAAGIDLEQKQYVWNMDHQPPSDDFVRTLADILAELHGTDQISAGQSGIEVIRPEDFRQMTADSMVDVKNKLGVSTTLWERWQKWVDD--------DAYWPGFSSLIHGDLHPPHILID-QNGRVTGLLDWTEAKVADPAKDFV---LYQTIFGEKETARLLEYYDQAGGRIWAKMQEHISEMQAAYPVEIAKLALQTQQEEHINMALEALGVTSD 4DBX Chain:A ((121-252)) --------------------------------------------------------------------------------------------------------------------------------KDLARFLSELHSIN-ISGFKSNLVL----DFREKINEDNKKIKKLL--SRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHY---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4DBX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 322 -47104 -146.29 -392.53 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -146.29 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.359

(partial model without unconserved sides chains): PDB file : Tito_4DBX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DBX-query.scw PDB file : Tito_Scwrl_4DBX.pdb: