Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIQLSKGQRIDLTKTNPGLTKAVIGLGWDTNKY-SGGHDFDLDASAFLVDA-HDNCVNDLDFVFYNNLEHPSGGVIHTGDNRTGEGDGDDEQIIVDFSKIPAHIEKIGITVTIHDAEARSQNFGQVSNAFVRVVDEETQNELLRFDLGEDFSIETAVVVCELYRHG-GEWKFNAIGSGFSGGLAALCRNYGLQV 2QZ7 Chain:A ((22-175)) ------------------GLKRVDVRLKWDPSPWDRPPHHLDIIATTYAADAPHGRPV----YVVQFDKRSPDGTINMSRHSRTGQGFGFVEEMTFELDRLSPSIARVIVGVAIHQDNGH-KTFDDVSNTGVVVA--EGYRELL-TDGFERVAGATAATVAEFTRNASGAWEFREAVRGF---------------

General information: TITO was launched using: RESULT: Template: 2QZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 791 -89518 -113.17 -592.83 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -113.17 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_2QZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QZ7-query.scw PDB file : Tito_Scwrl_2QZ7.pdb: