Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKFPKKLLPIAVLSSIAFSSLASGSVPEASAQEKKKGNQDEIKNVIVLIGDGMGVSYTSAYRYLKDNKKTKVVE--PTAFDQY----LVGQQTTYPDDPEQNVTDSAAAATAMSAGIKTYNNAIAVDNDGSEAKTVLEAAKEKGKATGLVATSEITHATPASFGSHDHSRKNMNSIADDYF----DEMVNG--KHKI--------------------DVLLGGGKSNFDRKDRNLIKEFKKAGYSYVDDRKDMLKNKDSQVLGLFADGGLPKKI---------DRTKDIPSLKDMTNTAIKKLNKDKDGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEQAYKAAIDFAKKDKHTLVVATADHSTGG-----------------------------YSIGA----DGIYN-------WFSEPIKAAK--RTPDFMAEKIADGADVEKT---LKTYIDQK--KLALTKAEIQSVEEAAKSKEVLDID-----NAIENIFNKRSHTG----WTTGGHTGEDVPVYAYGPSSETFAGQIDNTEIAKNVFKALQYNIKINDK 3E2D Chain:A ((2-482)) -----------------------------------------EIKNVILMIGDGMGPQQVGLLETYANQAPNSIYKGNKTAIYQLAQEGVIGSSLTHPEDA--IVVDSACSATMLATGIYSSSEVIGIDSQGNHVETVLEKAKKAGKATGLVSDTRLTHATPASFAAHQPHRSLENQIASDMLATGADVMLSGGLRHWIPKSTNDKGETYKQLEKLTQGDVYLKSKR----KDDRNLLTEAEKDGYQLAFNRNMLDDAKGDKLLGLFAYSGMDDGIAYSNKKKSGERTQ--PSLKEMTQKALNILSKDEDGFFLMVEGGQIDWAGHSNDAGTMLHELLKFDEAIQTVYEWAKDREDTIVIVTADHETGSFGFSYSSNDLPKPQKRSGEAFADRDYAPNFNFGAFDILDGLYNQKQSYYGMISEFQKLDKSLQTPEKLAEIVNKNSEFPITAEQAKNVLASKPNPYRLAQHKYLSAEEVPA---INDFDAFFPYNDRGNLLAREQATGQNIVWGTGTHTHTPVNVFAWGPAEKIL--------------------------

General information: TITO was launched using: RESULT: Template: 3E2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2220 -99789 -44.95 -259.87 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -44.95 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_3E2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E2D-query.scw PDB file : Tito_Scwrl_3E2D.pdb: