Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIKRALISVSDKTNLVPFVKELTELGVEVISTGGTKKLLQENGVDVIGISEVTGFPEIMDGRLKTLHPNIHGGLLAVRGNEEHMAQINEHGIQPIDLVVVNLYPFKETISKEDVTYEEAIENIDIGGPGMLRAASKNHQDVTVIVDPADYSPVLNQIKEEGSVSLQKKRELAAKVFRHTAAYDALIADYLTNVV-GEKEPEQFTVTFEKKQSLRYGENPHQEATFYQTALPVKGSIAQAEQLHGKELSYNNIKDADAAVQIVREFTEPAAVA-VKHMNPCGVGTGKTIAEAFDRAFEADKTSIFGGIIALNREVDKATAEALHNIFLEIIIAPSFSQEALDVLTAKKNLRLLTLDVSAAVQK--EKQLTSVQGGLLIQDLDMHGFDDAE-ISIPTKREPNEQEWEDLKLAWKVVKHVKSNAIVLAKDNMTVGVGAGQMNRVG---SAKIAIEQAGEKAKGSALGSDAYFPMPDTVEEAAKAGVTAIIQPGGSIRDEDSIKKADEYGIAMVFTGIRHFKH 4EHI Chain:A ((26-534)) ----RALLSVSDKEGIVEFGKELENLGFEILSTGGTFKLLKENGIKVIEVSDFT--------------------------------------ILGIDLVCVNLYPFKKTTIMSD-DFDEIIENIDIGGPAMIRSAAKNYKDVMVLCDPLDYEKVIETLK-KGQNDENFRLNLMIKAYEHTANYDAYIANYMNERFNGGFGASKFIVG-QKVFDTKYGENPHQKGALYEFDAFFS---ANFKALKG-EASFNNLTDINAALNLASSFDKAPAIAIVKHGNPCGFAIKENLVQSYIHALKCDSVSAYGGVVAINGTLDEALANKINEIYVEVIIAANVDEKALAVFEGKKRIKIFTQESPFLIRSFDKYDFKHIDGGFVYQNSDEVGEDELKNAKLMSQREASKEELKDLEIAMKIAAFTKSNNVVYVKNGAMVAIGMGMTSRIDAAKAAIAKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEPGGSIRDDEVVKAADEYGMALYFTGVRHFLH

General information: TITO was launched using: RESULT: Template: 4EHI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2354 -217707 -92.48 -470.21 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -92.48 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_4EHI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EHI-query.scw PDB file : Tito_Scwrl_4EHI.pdb: