Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISVLFVCLGNICRSPMAEAIFRDLAAKKGLEGKIKADSAGIGGWHIGNPPHEGTQEILRREGISFDGMLARQVSEQDLDDFDYIIAMDAENIGSLRSMAGFKNTSHIKRLLDYVEDSDLADVPDPYYTGN--FEEVCQLIKTGCEQLLASIQKEKQL 4LRQ Chain:A ((2-149)) -QKVLVVCMGNICRSPTAEAVLRAKAAQ--LKVDVEVDSAGTIGYHQGNPPDARSKAAGEKRGYSFSGIKARKIRDEDFVKFDWILAADQENLAELKARCPQSHQHKLSLMLSH-SDSEYQEIPDPYYGGERGFELVLDLVEDAAEQFLLKL------

General information: TITO was launched using: RESULT: Template: 4LRQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 798 -85381 -106.99 -584.80 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -106.99 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_4LRQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LRQ-query.scw PDB file : Tito_Scwrl_4LRQ.pdb: