Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEVGILPVHVLYVFFIGAIILFMLLRKDTTFISLFGIFIISLWASHSLSASVSSLFHSFIYAAGELLPTIFIICFIVSMSDLLTKTGINEAMISPFASLVRG-PVTAYWLIGGLMFAISLFFWPSPGVALIGAVLLPAAARAGLTPLA-----AAMAMNLFG---HGFALSGDFVIQAAPKLTADAAGIPVGDVVSASIPLVLIMGVTTTTAAFIMIQRERKKQEHPTSFSPVLSGEQDNSLYLPKRLRSILAF-LIPLAFLADIACMLLFN-L---QGNDATALIGGSAICILFTVHFFVYKHKGLEKITGYFIDGFKFGFKVFGPVIPIAAFFYLGDSGYESILGTSLPKGSHGIVNDLGIALSHMMP-MSKEL-AATALTAAGAITGLDGSGFSGISLAGSIAKLFSSALHADPAILTALGQISAIWVGGGTLVPWA---LIPAAAICKVDPFELARKNFIPVAIGLLVTTLAAVMML 4F35 Chain:D ((80-445)) ------------------------------------------------------------------ANSIIFLFLGGFALAAAMHHQGLDKVIADKVLAMAQGKMSVAVFMLFGVTALLSMWISNTATAAMMLPLVLGVLSKVDADKQRSTYVFVLLGVAYSASIGGIATLVGSPP----NAIAAAEVGLSFTDWMKFGLPTAMMMLPMAIAILYFLL-----------------------VNWDKGK-VVTLGIFGLTVFLWI-FSS-PINAALGGFKSFDTLVALGAIL------MLSFARV-VHWKEI----QKT----ADWGVLLLFGGG----------LCLSNVL--KQTGTSVFLANALSDMVSHMGIFVVILVVATFVVFLTEFA-SNTASAALLIPVFATVAEAFGMSPVLLSVLIAVAAS---CAFMLPVATPPNAIVFASGHIKQSEMMRVGLYLNIACIGLLTAIAM---

General information: TITO was launched using: RESULT: Template: 4F35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1678 -276858 -164.99 -831.41 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain D : 0.62 3D Compatibility (PKB) : -164.99 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_4F35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F35-query.scw PDB file : Tito_Scwrl_4F35.pdb: