Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTRKPFIICDFDGTITMNDNIINIMKTFAPPEWMA-LKDGVLSKTLSIKEGVGRMFGLLPSSLKEEITSFVLEDA-KIREGFREFVAFINEHEIPFYVISGGMDFFVYPLLEGI-VEKDRIYCNHASFDNDYIHIDWPHSCKGTCSNQCGCCKPSVIHELSEPNQY--IIMIGDSVTDVEAAKLSDLCFARDYLLNECREQNLNHLPY--QDFYEIRKEIENVKEVQEWLQNKNAGESSLK 1L8L Chain:A ((20-224)) ----------DVDSTVIREEGIDELAKICGVEDAVSEMTRRAMGGAVPFKAALTERLALIQPS-REQVQRLIAEQPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVASKLNIPATNVFANRLKF---YFNGEYAGFDETQPTAESGG-KGKVIKFLKEKFHFKKIIMIGDGATDMEACPPADAFIG--FGGNVIRQQVKDNAKWYITDFVELLGELE--------------------

General information: TITO was launched using: RESULT: Template: 1L8L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 923 -105025 -113.79 -530.43 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -113.79 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_1L8L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L8L-query.scw PDB file : Tito_Scwrl_1L8L.pdb: