Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVISGSKKGRSLKAVAGTSTRPTTDKVKESIFNMIGPYFDGGRGLDLFAGSGGLGIEALSRGFEHCIFVDRDFKAIQTVKSNLKTLELTKHAQVYRNDAERALHAAAKRETGFRGIFLDPPYKEQKLKALLTLIDEYQMLEEDGFIVAEHDREVELPETVGDLVMTRKETYGLTGVAIYKKRG 2FPO Chain:A ((14-191)) IRIIGGQWRGRKLPVPDS------TDRVRETLFNWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLK-AGNARVVNSNAMSFL---AQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEVENGLPTVPANWSLHREKVAGQVAYRLYQR--

General information: TITO was launched using: RESULT: Template: 2FPO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 924 -96750 -104.71 -562.50 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -104.71 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_2FPO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FPO-query.scw PDB file : Tito_Scwrl_2FPO.pdb: