Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIKRSFISISVLMVIFGLMISVQFNSLKHPKVRDTRDMWDIREELTSEQKKQEKLLAEI-NKYDKLLNSYSQTKEMT------KE--------TALNNTLQSLKKTAGMTDITGSGIVITISPLFSESLTGEPIENPPPDLLK-KLINELNSYGAEHISINERRVVNHTVIRDINGTTKIDGYALDDYPLTVKVLAKDPDMLHSRVKGSGLEDLFASENLALKAGKSESKLTLKAYDRPLDVQQLKLLKD 4TLM Chain:A ((149-275)) -------------------------------------------HEGRAAQKKLETLLEEKESKADKVLQFEPGTKNLTALLLEAKELEARVIILSASEDDATAVYKSAAMLDMTGAGYVWLVG---EREISGSALRYAPDGIIGLQLINGKNE--SAHIS-------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TLM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 253 2918 11.53 30.40 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 11.53 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.267

(partial model without unconserved sides chains): PDB file : Tito_4TLM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TLM-query.scw PDB file : Tito_Scwrl_4TLM.pdb: