Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNAKQEHFELDQEWVELMVEAKEANISPEEIRKYLLLNKKSAHPGPAARSHTVNPF 1Q08 Chain:A ((2-30)) -----------DLQRLKFIRHARQLGFSLESIRELLSIRI-----------------

General information: TITO was launched using: RESULT: Template: 1Q08.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 36 -8784 -244.00 -302.90 target 2D structure prediction score : 0.93 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -244.00 2D Compatibility (Sec. Struct. Predict.) : 0.93 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 1.030

(partial model without unconserved sides chains): PDB file : Tito_1Q08.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Q08-query.scw PDB file : Tito_Scwrl_1Q08.pdb: