Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVANDYEKRDNNSYYVDH--GSEGTNITRDNDGFFEETAAEIAEPYRAADRSNDQDNDRSGGNVNEGRGIGYIALALSIISLFVLPVLLGAAGIIVGYIARRRGAQG--LGAWAMGIGVVSLVLGIFIIPFF 3SIH Chain:A ((136-226)) ------FHHRQRDPLYSDHLIYSPGVPVFRDDAGRLLE------EPYRVAFLTSPAPNRRAIGDLRTVEEIGR--------------VLRGRAAKVLA-AARHHGHRRLVLGAWGCGV---------------

General information: TITO was launched using: RESULT: Template: 3SIH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 199 17943 90.16 206.24 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 90.16 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.232

(partial model without unconserved sides chains): PDB file : Tito_3SIH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SIH-query.scw PDB file : Tito_Scwrl_3SIH.pdb: