Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENKIIYFLCTGNSCRSQMAEGWAKQYLGDEWKVYSAGIEAHGLNPNAVKAMKEVGIDISNQTSDIIDSDILNNADLVVTLCGDAADKCPMTPPHVKREHWGFDDPARAQGTEEEKWAFFQRVRDEIGNRLKEFAETGK 1Z2E Chain:A ((1-139)) MENKIIYFLCTGNSCRSQMAEGWAKQYLGDEWKVYSAGIEAHGLNPNAVKAMKEVGIDISNQTSDIIDSDILNNADLVVTLCGDAADKCPMTPPHVKREHWGFDDPARAQGTEEEKWAFFQRVRDEIGNRLKEFAETGK

General information: TITO was launched using: RESULT: Template: 1Z2E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 750 -50394 -67.19 -362.55 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -67.19 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.603

(partial model without unconserved sides chains): PDB file : Tito_1Z2E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z2E-query.scw PDB file : Tito_Scwrl_1Z2E.pdb: