Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLITPDELKSYSVFESVKTRPDELLKQDILEAT-----ADIILKVGHDFSDAEYIPLP-----ETVRLALLKLSQFYALINGDESI-IKGYTTEKIGDYSYTLGDGSSLQKPDVYALIKDYVKPADPDLEGIEAKVRMRSI 4G07 Chain:A ((269-362)) -ILMTNDEAFAHAVEEAVERQLH----TETASASWRDFGAVILVK---DFEDA--IPLANRIAAEHLEIAVADAEAFVPRIRNAGSIFIGGYTPEVIGDY------------------------------------------

General information: TITO was launched using: RESULT: Template: 4G07.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 210 -13269 -63.19 -167.96 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -63.19 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.262

(partial model without unconserved sides chains): PDB file : Tito_4G07.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G07-query.scw PDB file : Tito_Scwrl_4G07.pdb: