Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISTKGRYGLTIMIELAKKHGEGPTSLKSIAQTNNLSEHYLEQLVSPLRNAGLVKSIRGAYGGYVLGSEPDAITAGDIIRVLEGPISPVEVLEDEEPAKRELWIRIRDAVKEVLDSTTLEDLASYTDGEQEAYMFYI 3T8R Chain:A ((4-129)) ---STKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQLVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGP----ESIESEPPAQKQLWIRMRDAVRDVLDNTTLKYLAEYVDT---------

General information: TITO was launched using: RESULT: Template: 3T8R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 431 -53396 -123.89 -437.67 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -123.89 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_3T8R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T8R-query.scw PDB file : Tito_Scwrl_3T8R.pdb: