Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTKKQQYVTIKGTKNGLTLHLDDACSFDELLDGLQNMLSIEQYTDGKGQKISVHVKLGNRFLYKEQEEQLTELIASKKDLFVHS----IDSEVITKKEAQQIREEAEIISVSKIV----RSGQVLQVKGDLLLIGDVNPGGTVRAGGNIFVLGSLKGIAHAGFNGNNQAVIAASEMLPTQLRINHVLNRSPDHIQKGNEMECAYLDTDGN-MVIERLQHLAHLRPDLTRLEGGM 1HF2 Chain:A ((2-206)) --------VDFKMTKEGLVLLIKDYQNLEEVLNAISARITQMGGFFAKGDRISLMIENHNK-----HSQDIPRIVSHLRNLGLEVSQILVG----------KVQSRTTVESTGKVIKRNIRSGQTVVHSGDVIVFGNVNKGAEILAGGSVVVFGKAQGNIRAGLNEGGQAVVAALDLQTSLIQIAGFITHS-----KGEENVPSIAHVKGNRIVIEPFDKVSF------------

General information: TITO was launched using: RESULT: Template: 1HF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1001 -41849 -41.81 -224.99 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -41.81 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_1HF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HF2-query.scw PDB file : Tito_Scwrl_1HF2.pdb: