Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREDTKVYDITIIGGGPVGLFTAFYGGMRQASVKIIESLPQLGGQLSALYPEKYIYDVAGFPKIRAQELINNLKEQMAKFDQTICLEQAVESVEKQADGVFKLVTNEETHYS-KTVIITAGNGAFKPRKLELENAEQYEGKNLHYFVDDLQKFAGRRVAILGGGDSAVDWALMLEPIAKEVSIIHRRDKFRAHEHSVENLHAS----KVNVLTPFVPAELIGEDKIEQLVLEEVKGDRKEILEIDDLIVNYGFVSSLGPIKNWGLDIEKNSIVVKSTMETNIEGFFAAGDICTYEGKVNLIASGFGEAPTAVNNAKAYMDPKARVQPLHSTSLFENK 2ZBW Chain:A ((7-328)) --------DVLIVGAGPTGLFAGFYVGMRGLSFRFVDPLPEPGGQLTALYPEKYIYDVAGFPKVYAKDLVKGLVEQVAPFNPVYSLGERAETLEREGD-LFKVTTSQGNAYTAKAVIIAAGVGAFEPRRIGAPGEREFEGRGVYYAVKSKAEFQGKRVLIVGGGDSAVDWALNLLDTARRITLIHRRPQFRAHEASVKELMKAHEEGRLEVLTPYELRRVEGDERVRWAVVFHNQTQEELALEVDAVLILAGYITKLGPLANWGLALEKNKIKVDTTMATSIPGVYACGDIVTYPGKLPLIVLGFGEAAIAANHAAAYANPALKVNPGHSS------

General information: TITO was launched using: RESULT: Template: 2ZBW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1839 -53549 -29.12 -168.92 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -29.12 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_2ZBW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZBW-query.scw PDB file : Tito_Scwrl_2ZBW.pdb: