Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVLKRIRDMFVASVHEGLDKMENPKVMLNQYVRDMESDIAKAKQTIVKQHTIAYQFKKKYEEAAEVAGKRKNQAQLAFDAGEEELAKKALTEMKYLEGKAAEHKASYEQANSQLAD--LKEQLAALETKLQDVKDKKQALIARANAAKAKEHMNTTFDKIDSESAYREFLRIENRIEEMEIRANYSKSAEAGTELTRKE---FADDVEAEIEKMRTLSLQKSDQTKAANE 5F97 Chain:A ((214-337)) ------------------------------------------------------------------------------------------TTTQTIDGKTVSTTISSKVVDSTAADNTYKQSYTEITNALKDVPDSAQALLAQA---------STLINTINTACPY---FSVTN------------KSGGPQMEPTRGKLCGFTEEISA-IQKMITDAQELVNQTSVINE

General information: TITO was launched using: RESULT: Template: 5F97.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 254 31596 124.39 287.23 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 124.39 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.209

(partial model without unconserved sides chains): PDB file : Tito_5F97.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F97-query.scw PDB file : Tito_Scwrl_5F97.pdb: