Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTASKFSVMFFMLLALCGCWDVKDIEQLSFARGLAIDETNDHQYKLTYQNLLPQSEDSQASGKPEFVNVTSHGKTILEAVSDVSIKDPPVYSDHLKVILLGEKLMRNQNVDQVLNHFIRDDELRRSSYLMAARGNAADVFTKGNPNQQQPMPSEKLIDLTTHSGYNGKIMIPLRIGRA-----SVYSQNGYSYLIQAVKNEKGKAKYDGAGIIKRGSNKLVG--FLSADETQTLSWVMGTIQGGVMPTTDKGH------PITFEIKKSKTKIKPVIENGKPVFHISVKTKGILTEDQNPNENSFSKSYLHRLENIFEKKLERDVKQVMDKLQHEYKTDPVFLSDHIRIQHPDYWNKVKGHWDEIFSETDFKYDISFKIINFGTVGK 1W2T Chain:A ((195-297)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------VRIGEKDILIYSITSTNSVLFSMGELK--EGKLNVEKRGLLDHGTDFYAAQTFFGTDRVVVIGWLQSWLRTGLYPTKREGWNGVMSLPRELYVENNELKVKPVDE-------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1W2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 309 22121 71.59 245.79 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 71.59 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.093

(partial model without unconserved sides chains): PDB file : Tito_1W2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W2T-query.scw PDB file : Tito_Scwrl_1W2T.pdb: