Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVFDVHKFDMKKEQDFLQVQFNLKNRINLSPTIHPDSINTCAGVDLAYWEQDGEPYGVCCIIVIDADTKEVIEKVHSMGRISVPYVSGFLAFRELPLIIEAAKKLETEPDVFLFDGNGYLHYNHMGVATHAAFFLGKPTIGIAKTYLKIKGCDFVTPEIEVGAYTDIIIDGEVYGRALRTRRDVKPIFLSCGNYIDLDSSYQITMSLINQESRLPIPVRLADLETHVLRTFYQKNHV 4B20 Chain:A ((1-221)) MDYRQLHRWDLPPEEA-IKVQNELRKKIKLTP--YEGEPEYVAGVDLSF---PGKEEGLAVIVVLEYPSFKILEVVSERGEITFPYIPGLLAFREGPLFLKAWEKLRTKPDVVVFDGQGLAHPRKLGIASHMGLFIEIPTIGVAKSRLY---GTFKMPEDKRCSWSYLYDGEEIIGCVIRTKEGSAPIFVSPGHLMDVESSKRLIKAFTLPGRRIPEPTRLAHIYTQRLK--------

General information: TITO was launched using: RESULT: Template: 4B20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1302 -156769 -120.41 -709.36 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -120.41 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_4B20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4B20-query.scw PDB file : Tito_Scwrl_4B20.pdb: