Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYAISSAADIWDHI-----SESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCGSPASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 2B8T Chain:A ((11-200)) ------IGWIEFITGPMFAGKTAELIRRLHRLEYADVKYLVFKPKIDTR-SIRNIQSRTGTSLPSVEVESAPEILNYIMSNSFNDETKVIGIDEVQFFDDRICEVANILAENGFVVIISGLDKNFKGEPFGPIAKLFTYADKITKLTAICNECGAEATHSLRKIDGKHADYNDDIVKIGCQEFYSAVCRHHHKVPNR---

General information: TITO was launched using: RESULT: Template: 2B8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 882 -21728 -24.63 -117.45 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -24.63 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.387

(partial model without unconserved sides chains): PDB file : Tito_2B8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B8T-query.scw PDB file : Tito_Scwrl_2B8T.pdb: