Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKGLKIVTIGGGSS-YTPELVEGFIKRYDELPVRELWLVDIPEGEEKLNIVGTLAKRMVEKAGVPIDIHLTLDRRKALKDADFVTTQFRVGLLQARAKDERIPLKYGVIGQETNGPGGLFKGLRTIPVILEIAKDIEELCPNAWLVNFTNPAGMVTEALLRYSNLKKVVGLCNVPIGIKMGVAKALDVD-VDRVEVQFAGLNHMVFGLDVFL-DGVSVKEQVIEAMGDPK-NAMTMKN-ISG-AEWEPDFL-KALNVIPCGYHRYYFKTKEMLEHELEASQTEGTRAEVVQKV-EKELFELYKDPNLAIKPPQLEKRGGAYYSDAACNLISSIYNDKHDIQPVNTINNGAIASIPDDSAVEVNCVMTKTGPKPIAVGDLPVSVRGLVQQIKSFERVAAEAAVTGDYQTALLAMTINPLVPSDTVAKQILDEMLEAHKAYLPQFFNKIEA 1U8X Chain:X ((26-465)) -KKSFSIVIAGGGSTFTPGIVLMLLDHL-EEFPIRKLKLYDN--DKERQDRIAGACDVFIREKAPDIEFAATTDPEEAFTDVDFVMAHIRVGKYAMRALDEQIPLKYGVVGQETCGPGGIAYGMRSIGGVLEILDYMEKYSPDAWMLNYSNPAAIVAEATRRLRPNSKILNICDMPVGIEDRMAQILGLSSRKEMKVRYYGLNHFGWWTSIQDQEGNDLMPKLKEHVSQYGYIPKT---SWNDTFAKARDVQAADPDTLPNTYLQYYLFPDDMVKKSN----PNHTRANEVMEGREAFIFSQCDMITREQSSENS-EIKIDDHASYIVDLARAIAYNTGERMLLIVENNGAIANFDPTAMVEVPCIVGSNGPEPITVGTIPQFQKGLMEQQVSVEKLTVEAWAEKSFQKLWQALILSKTVPNARVARLILEDLVEANKDFWPELD-----

General information: TITO was launched using: RESULT: Template: 1U8X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 2444 -66 -0.03 -0.15 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain X : 0.75 3D Compatibility (PKB) : -0.03 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_1U8X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U8X-query.scw PDB file : Tito_Scwrl_1U8X.pdb: