Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEKKNIYPNKEGCPVEFTLDVIGGKWKGILFYHMIDGKKRFNEFRRICPSITQRMLTLQLRELEADGIVHREVYHQVPPKVEYSLTEFGRTLEPIVLQMKEWGESNRDVLESYRSNGLVKDQQK 2HZT Chain:A ((4-98)) ---------------VEATLEVIGGKWK-VIL-HLTHGKKRTSELKRLMPNITQKMLTQQLRELEADGVINRIVYNQVPPKVEYELSEYGRSLEGILDML-AWGANHINR---------------

General information: TITO was launched using: RESULT: Template: 2HZT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 269 -39239 -145.87 -426.51 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -145.87 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_2HZT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HZT-query.scw PDB file : Tito_Scwrl_2HZT.pdb: