Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVYQTKRDVPVTLMIVFLILLIQADAIVPFVLGNMRVSGWIIFILLTLLNGLIIWSFIDLKYVLKEHHLIIKAGLIKHQIPYENIDKVVQKKKLWSGFRLIGSRHAITIYYQGGWGHAVISPQKSEEFIHKLKEKNSNIIIFTKSK 4POG Chain:A ((103-162)) --------------------------------------------------------------------------LMVKDIGAEHINKLIQVEGIVTRVGEIKPFVSVAVFVCKDCGHEMIVPQKPYESLEKVKK------------

General information: TITO was launched using: RESULT: Template: 4POG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 82 -7975 -97.25 -132.91 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -97.25 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.53 QMean score : -0.018

(partial model without unconserved sides chains): PDB file : Tito_4POG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4POG-query.scw PDB file : Tito_Scwrl_4POG.pdb: