Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPSLDNTADEKPAIDPILLKVLDAVPFRLSIDDGIEAVRQRLRD----LPRQPVHPELRVVDLAIDGPAGPIGTRIYWPPTCPDQAEAPVVLYFHGGGFVMGDLDTHDGTCRQHAVGADAIVVSVDYRLAPEHPYPAAIEDAWAATRWVAEHGRQVGADLGRIAVAGDSAGGTIAAVIAQRARDMGGPPIVFQLLWYPSTLWDQSLP--SLAENADAPILDVKAIAAFSRWYAGEIDLHNPPAPMAPGRAENLADLPPAYIAVAGYDPLRDDGIRYGELLAAAGVPVEVHNAQTLVHGYVGYAGVVPAATEATNRGLVALRVVLHG 1U4N Chain:A ((3-309)) -------------LDPVIQQVLDQLN-RMPAPDYKHLSAQQFRSQQSLFPPVKKEPVAEVREFDMDLPGRTLKVRMYRPEGV--EPPYPALVYYHGGGWVVGDLETHDPVCRVLAKDGRAVVFSVDYRLAPEHKFPAAVEDAYDALQWIAERAADFHLDPARIAVGGDSAGGNLAAVTSILAKERGGPALAFQLLIYPSTGYDPAHPPASIEENAEGYLLTGGMSLWFLDQYLNSLEELTHPW-FSPVLYPDLSGLPPAYIATAQYDPLRDVGKLYAEALNKAGVKVEIENFEDLIHGFAQFYSLSPGATKALVRIAEKLRDAL--

General information: TITO was launched using: RESULT: Template: 1U4N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -196701 for 2648 contacts (-74.3/contact) + 2D Compatibility (PS) -32743 + (NN) -20251 + (LL) 0 1D Compatibility (HY) -17600 + (ID) 6400 Total energy: -273695.0 ( -103.36 by residue) QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_1U4N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U4N-query.scw PDB file : Tito_Scwrl_1U4N.pdb: