Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFRLLVLMGFTFFFYHLHASGNLTKYINMKYAYLSFIAIFLLAILTAVQAYLFIKSPEKSGHHHDHDCGCGHDHEHDHEQNKPFYQRYLIYVVFLFPLVSGIFFPIATLDSSIVKTKGFSFKAMESGDHYSQTQYLRPDASLYYAQ------DSYDKQMKQLFNK---YSSKKEISLTDDDFLKGMETIYNYPGEFLGRTIEFHGFAYKGNAINKNQLFVLRFGIIHCIADSGVYGMLVEFPKDMDIKDDEWIHIKGTLASEYYQPFKSTLPVVKVTDWNTIKKPDDPYVYRGF 5B0O Chain:E ((5-139)) --------------------------------------------------------------------------------------------------------PIHARMQQLVSEFQNTLDALDSVIASRLMQMALEAARQVIGQTPAVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQR--------VEEMLGATLSLHGWRLRGDP-------TLHHGGCKVSADEG------DLDASVATRWQELCRLAAPGV----------------------------------

General information: TITO was launched using: RESULT: Template: 5B0O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 357 -35459 -99.32 -281.42 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain E : 0.56 3D Compatibility (PKB) : -99.32 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.013

(partial model without unconserved sides chains): PDB file : Tito_5B0O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5B0O-query.scw PDB file : Tito_Scwrl_5B0O.pdb: