Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLSLFIIAVLMPVILLSACSDHAEEHASINTKKTVENITDVRKTAKTSIDWTKPSGGEYPDIKQKHVWIDVNVKEQKAYIKEGS-NTIYTMMISSGLDQTKDDATPKG-TFYVEPERGEWFFSEG----------------------YQEGAEYWVSWKNHGEFLFHSVPMTKDQKVIKTEAEKLGTKASHGCIRLTIPDAKWVYENIPEHTKVVIS 4LZH Chain:A ((95-232)) -----------------------------------------------------------------VHEGIIINSAEMRLYYYPKGTNTVIVLPIGIG---------PINWTTKVERKKAGPTWTPTAKMHAEYAAAGNPLPAVVPAGPDNPMGLYALYIG--RLYAIHGTNAN----------FGIGLRVSHGCVRLRNDDIKFLFENVPVGTRVQFI

General information: TITO was launched using: RESULT: Template: 4LZH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 533 -73187 -137.31 -677.66 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -137.31 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.342

(partial model without unconserved sides chains): PDB file : Tito_4LZH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LZH-query.scw PDB file : Tito_Scwrl_4LZH.pdb: