Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIRQLRYFITIAQEQKITSAAKKLHMAQPPLSRQLKQLEDELGVVLFDRNKKKQMTLTYEGAVFLKRAKEILHRFEDAVIEVQELKEEVAGTLAVGSTIYCA-ALMLEKVTQIKERYPHLTFNIWENEPATLLELLESRQIDAAVTTTLIKSDTVQFKQLDDIPCVLVLSDEAGYPCGDTIKMVDIPSFPLILLRPVNGKGVYNQVMNEFHRL---NLEPRIVCECHDSATLLSLVSSGFGASILPVTMIPVHM-R-NHVHTVHIENNPFIMTPAVMWRTDSYLPKPAQQFLDLF 4X6G Chain:A ((7-299)) MTLTELRYIVTLAQEQHFGRAAERCHVSQPTLSVGVKKLEDELGVLIFERS-KSAVRLTPVGEGIVAQAQKVLEQAQGIRELAQAGKNQLAAPLKVGAIYTIGPYLFPHLIPQLHRVAPQMPLYIEENFTHILRDKLRTGELDAIIIALPFQEADVLTKPLFDEPFYVLMPADHPWTAKASIDSELLNDKSLLLLG--EGHDFRDQVLEACPTVRKGDENKHTTVESSSLETIRHMVASGLGVSVLPFSAVDSHHYAPGVIEVRPFSAPVPFRTVAIAWRASFPRPRAIEVLADSI

General information: TITO was launched using: RESULT: Template: 4X6G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1260 -208290 -165.31 -725.75 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -165.31 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_4X6G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X6G-query.scw PDB file : Tito_Scwrl_4X6G.pdb: