Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MLKKIALTLCPAIVGSLLFFTAPASAHVSVKPAESAAGSWETYTMKVPSEKNLPTTKVVLKMPKDVEFQQ--YEPIPGWKVSTQKHDDKSVSVTWEATDGGIQEGQFQQFTFVAKNPDKAEEAAWDAYQYYKDGSIVEWTGDEDADTPHSITNITSAKQVTDEHGATKTEDDSENSGSSALDITAMVLSA-AAIILSVAALVKKKRA- 4O6Y Chain:A ((10-219)) PIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLILFNGEAMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYS-----LHSWLGLACLFLFAFQWAAGFVTY----WYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVITRYSTEAMLVNTMGVLILILGGFVILGVVT

General information: TITO was launched using: RESULT: Template: 4O6Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 697 -58410 -83.80 -299.54 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -83.80 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.59 QMean score : -0.079

(partial model without unconserved sides chains): PDB file : Tito_4O6Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O6Y-query.scw PDB file : Tito_Scwrl_4O6Y.pdb: